OrangerV1, Main, Exploration, bibRecord, 004165

Invertase and sucrose synthase activity, carbohydrate status and endogenous IAA levels during Citrus leaf development

Identifieur interne : 004165 ( Main/Exploration ); précédent : 004164; suivant : 004166

Invertase and sucrose synthase activity, carbohydrate status and endogenous IAA levels during Citrus leaf development

Auteurs : Arthur A. Schaffer [Israël] ; Oded Sagee [Israël] ; Eliezer E. Goldschmidt [Israël] ; Raphael Goren [Israël]

Source :

Physiologia Plantarum [ 0031-9317 ] ; 1987-01.

RBID : ISTEX:91A6B48642C6440288DF4A3702B34C27FF0390FF

Descripteurs français

Pascal (Inist)
- Agrume, Auxine, Catabolisme, Citrus sinensis, Développement, Enzyme, Feuille végétal, Plante fruitière, Saccharose, Substance croissance végétal, Sucrose synthase, β-D-Fructofuranosidase.
Wicri :
- topic : Agrume, Enzyme, Saccharose.

English descriptors

KwdEn :
- Auxin, Catabolism, Citrus fruit, Citrus sinensis, Development, Enzyme, Fruit crop, Plant growth substance, Plant leaf, Sink activity, Sucrose, Sucrose synthase, sucrose metabolism, β-D-Fructofuranosidase.

Abstract

Levels of activity of the sucrose catabolizing enzymes, acid invertase (EC 3.2.1.26) and sucrose synthase (EC 2.4.1.13), were measured during development of new leaves of Citrus sinensis (L.) Osbeck cv. Shamouti. Soluble acid invertase showed a peak activity of 32 nkat (g fresh weight)−1 at ca 60% of full leaf expansion and rapidly declined toward and after full expansion. There was no concomitant increase in an insoluble form of the enzyme. Sucrose synthase activity, measured in the synthesis direction, declined from 33% of full leaf expansion [10 nkat (g fresh weight)−1] 10, and following, full expansion. Highest sucrose synthase activity, measured in the cleavage direction, was 6 nkat (g fresh weight)−1 and showed little change during development. Acid invertase has a Km of 5 mMfor sucrose, while sucrose synthase had a Km of 118 mM for sucrose. Changes in acid invertase activity correlated with changes in the reducing sugar:sucrose ratio. These results suggest that soluble acid invertase activity is the primary enzyme responsible for sucrose catabolism in the expanding Citrus leaf. Changes in leaf expansion rate and invertase activity did not correlate positively with changes in endogenous free IAA level, as determined by enzyme linked immunoassay.

Url:

https://api.istex.fr/document/91A6B48642C6440288DF4A3702B34C27FF0390FF/fulltext/pdf

DOI: 10.1111/j.1399-3054.1987.tb01959.x

Affiliations:

Israël

Le document en format XML

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<front><div type="abstract" xml:lang="en">Levels of activity of the sucrose catabolizing enzymes, acid invertase (EC 3.2.1.26) and sucrose synthase (EC 2.4.1.13), were measured during development of new leaves of Citrus sinensis (L.) Osbeck cv. Shamouti. Soluble acid invertase showed a peak activity of 32 nkat (g fresh weight)−1 at ca 60% of full leaf expansion and rapidly declined toward and after full expansion. There was no concomitant increase in an insoluble form of the enzyme. Sucrose synthase activity, measured in the synthesis direction, declined from 33% of full leaf expansion [10 nkat (g fresh weight)−1] 10, and following, full expansion. Highest sucrose synthase activity, measured in the cleavage direction, was 6 nkat (g fresh weight)−1 and showed little change during development. Acid invertase has a Km of 5 mMfor sucrose, while sucrose synthase had a Km of 118 mM for sucrose. Changes in acid invertase activity correlated with changes in the reducing sugar:sucrose ratio. These results suggest that soluble acid invertase activity is the primary enzyme responsible for sucrose catabolism in the expanding Citrus leaf. Changes in leaf expansion rate and invertase activity did not correlate positively with changes in endogenous free IAA level, as determined by enzyme linked immunoassay.</div>
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Invertase and sucrose synthase activity, carbohydrate status and endogenous IAA levels during Citrus leaf development

Invertase and sucrose synthase activity, carbohydrate status and endogenous IAA levels during Citrus leaf development

Source :

Descripteurs français

English descriptors

Abstract

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